Prep HPLC Column, RP C12, 10um, 100A. 30.0mm ID x 250mm Length. Cogent Brand. 1 EA.
Additional Information:
Cogent RP C12 is a unique reversed-phase preparative HPLC stationary phase based on an adamantane bonded ligand, a rigid diamond-like carbon structure that provides distinctive chromatographic selectivity compared to traditional alkyl phases. The combination of high-purity silica, a narrow particle size distribution, low metal content, complete double endcapping, and the rigid bonded phase design makes this column particularly effective for purifying complex mixtures while supporting rapid equilibration between runs. The phase generally provides retention that falls between C8 and C18 columns, making it an excellent choice when C18 retention is excessive and C8 retention is insufficient.
68012-P30-250 is a preparative HPLC column with dimensions of 30 mm ID x 250 mm length, packed with 10 µm RP C12 particles featuring a 100 Å pore size. The large internal diameter provides exceptionally high sample loading capacity for preparative purification, while the 250 mm length delivers maximum resolving power and peak capacity for demanding separations. The 10 µm particle size supports higher flow rates with lower operating backpressure, making this column well suited for purification, fraction collection, process development, and method scale-up. Typical operating flow rates are approximately 30–80 mL/min depending on sample load, mobile phase composition, and system capabilities.
Compatible with reversed-phase mobile phases, including 100% aqueous conditions, this column maintains reproducible retention without loss of performance between runs. The unique adamantane bonded phase can provide valuable selectivity advantages when purifying hydrophobic and moderately hydrophobic compounds from complex sample matrices. The extended column length maximizes separation efficiency and can improve purification of closely related compounds, helping achieve higher purity fractions in demanding preparative workflows. A specialized preparative HPLC system capable of higher flow rates, larger injection volumes, and fraction collection is required. This product is supplied as a Special Order Item manufactured specifically upon receipt of order.
Key Benefits
Unique adamantane-based RP C12 stationary phase
Fully double endcapped chemistry
High-purity silica with low metal content
Narrow particle size distribution
Compatible with 100% aqueous mobile phases
Rapid equilibration between runs
Excellent for complex mixture purification
Retention between C8 and C18 phases
Exceptionally high sample loading capacity
Maximum resolving power with 250 mm column length
Typical Applications
Preparative HPLC purification
Fraction collection
Compound isolation
Pharmaceutical research
Natural product purification
Synthetic chemistry
Process development
Method scale-up
Production support purification
Complex mixture purification
Specifications
Product Type: Preparative HPLC Column
Stationary Phase: RP C12
Chromatography Mode: Reversed Phase
Bonded Phase: Adamantane-Based C12 Ligand
Endcapping: Fully Double Endcapped
Particle Size: 10 µm
Pore Size: 100 Å
Column Dimensions: 30 mm ID x 250 mm
Length: 250 mm
Typical Flow Rate: 30–80 mL/min
Brand: Cogent
Additional Resources
Special Order Item Information
Important Notes
Intended for purification and fraction collection rather than routine analytical HPLC
Compatible with 100% aqueous mobile phases
Excellent choice when C18 retention is too great and C8 retention is insufficient
Rapid re-equilibration can improve preparative throughput
Exceptionally high loading capacity supports preparative-scale isolations
Extended column length can maximize separation of closely related compounds
Requires a preparative HPLC system capable of higher flow rates and fraction collection
Special Order Item